The genetic expression LGI3 (Leucine rich repeat LGI family member 3) among different NSLC cell lines and its effect on proliferation of cancer cells

초록

LGI3 as proinflammtory cytokines in the lungs with associate signaling proteins may be related with prognosis especially in nonsmall cell lung cancer(NSCLC). Our recent meta-analysis reported the prognostic role of LGI3 in patients with NSCLC. The aim of this study was to define the expressional levels among various kinds of NSCLC cell lines for the pilot study for further experimental investigations. We used 14 NSCLC cell lines purchased from NCI and Korean cell banks. The level of LGI3 transcript was compared using quantitative RT-PCR. Western blot analysis was performed in order to detect LGI3 protein in the whole cell lysate. ELISA was done in both cells and culture medium. Cellular proliferation assay was done after treatment of LGI3 protein to A549 cells by MTS assay. LGI3 level was also checked with human serum. The most abundant mRNA level of LGI3 was observed in NCI-H460 and NCI-H1650 lung adenocarcinoma cells. Notably, NCI cell lines showed higher expression of LGI3 than those of Korean-derived cell lines, which suggested the ethnic difference in NSCLC such as adenocarcinoma. A549 cell growth was suppressed by the treatment of LGI3 protein, which suggest that LGI3 might play a role as a tumor suppressor gene or negative autoregulatory feedback. The expressional levels of LGI3 were different among different cell lines, especially between Caucasian and oriental patient cell lines. Further investigation might be necessary about molecular interactions and role of prognosis and pathogenesis.

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