ScholarWorks@중앙대학교

초록

The presence of excessive hydrogen peroxide (H2O2) in the body plays a key role in inflammatory diseases. Among the various methods available, fluorescent nanoparticles have been used to detect H2O2 in living cells. Herein, the design of CD44-targetable fluorescent nanoparticles is presented for intracellular H2O2 imaging in inflammatory macrophages. Accordingly, CD44-targetable hyaluronic acid-histidine (HA-His) conjugates were sequentially reacted with permanganate and fluorescein (FL) to synthesize HA-His/MnO2/FL nanoparticles. These prepared HA-His/MnO2/FL nanoparticles exhibited good dispersibility in water and 10% fetal bovine serum solution. Owing to their rapid responsiveness to H2O2, the HA-His/MnO2/FL nanoparticles showed a good linear relationship with H2O2 concentration (0-100 mu M). Moreover, the CD44-targetable HA-His/MnO2/FL nanoparticles were readily internalized in lipopolysaccharide-induced inflammatory macrophages, allowing successful imaging of intracellular H2O2 in inflammatory macrophages.

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