ScholarWorks@중앙대학교

초록

Two Gram-stain-negative, obligately aerobic, non-motile rod-shaped bacteria, designated strains W23T and W31T, exhibiting catalase- and oxidase-positive activities, were isolated from the phycosphere of a marine red alga. Strain W23ᵀ grew optimally at 25 °C, pH 8.0-9.0, and 3.0-4.0 % (w/v) NaCl, whereas strain W31T grew optimally at 25-30 °C, pH 8.0, and 3.0-4.0 % NaCl. Ubiquinone-8 was the major respiratory quinone in both strains. Strain W23T predominantly contained C16:0, summed features 3 (C16:1ω7c and/or C16:1ω6c) and 8 (C18:1ω7c and/or C18:1ω6c), and C12:0 as major fatty acids, with phosphatidylglycerol and phosphatidylethanolamine as main polar lipids. In contrast, strain W31T exhibited isoC15:0, summed feature 1 (isoC15:1H and/or C13:0 3-OH), C12:0, and isoC11:0, with diphosphatidylglycerol, phosphatidylethanolamine, and phosphatidylmonomethylethanolamine as dominant polar lipids. The genomic DNA G + C contents were 44.5 % for strain W23T and 42.3 % for strain W31T. Phylogenetic, genomic, and phenotypic analyses support the classification of strains W23ᵀ and W31ᵀ as novel species within the genera Ningiella and Marinicella, for which the names Ningiella algicola sp. nov. (W23T = KACC 23126T = JCM 35964T) and Marinicella algicola sp. nov. (W31T = KACC 23129T = JCM 35965T) are proposed. Genome-based phylogenomic analyses also supported the reclassification of Alteromonas lipolytica, Alteromonas alba, and Alteromonas oceani into the genus Marisediminitalea; Alteromonas sediminis into a new genus, Paralteromonas; and Alteromonas flava, Alteromonas facilis, and Alteromonas arenosi into another new genus, Neoalteromonas. Furthermore, Methylophaga aminisulfidivorans is reclassified as a later heterotypic synonym of Methylophaga thalassica. Copyright © 2025 Elsevier GmbH. All rights reserved.

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