Immunohistochemical study of the distribution of neuronal voltage-gated calcium channels in the nNOS knock-out mouse cerebellum

Kim, M.J.; Chung, Y.H.; Joo, K.M.; Oh, G.T.; Kim, J.; Lee, B.; Cha, C.I.

doi:10.1016/j.neulet.2004.07.047

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Immunohistochemical study of the distribution of neuronal voltage-gated calcium channels in the nNOS knock-out mouse cerebellum

Kim, M.J.;
Chung, Y.H.;
Joo, K.M.;
Oh, G.T.;
Kim, J.;
외 2명

Citations

SCOPUS

10

초록

Nitric oxide (NO) participates in synaptic plasticity, neuronal development, and apoptosis. The involvement of NO and ionic calcium in synaptic plasticity imply that NO may exert an effect on Ca 2+ channels. Therefore, we investigated changes in the expressions of calcium channel subunits (Ca v1.2/α 1C, Ca v1.3/ α 1D, Ca v2.1/α 1A, and Ca v2.2/α 1B) in nNOS knock-out (-/-) (nNOS (-/-)) mouse cerebellum using an immunohistochemical approach. We found that the immunoreactivities of the Ca v1.2 and Ca v1.3 subunits were reduced in the cell bodies of Purkinje cells in these mice and that the signal of the Ca v1.2 subunit in neurons and of the Ca v1.3 subunit in the neuropils of nNOS (-/-) mice cerebellar nuclei were significantly down-regulated. We show, for the first time, that prolonged NO deficiency in the cerebellum may affect calcium channel protein expressions, especially, of the Ca v1.2 and Ca v1.3 subunits. © 2004 Elsevier Ireland Ltd. All rights reserved.

키워드

Calcium channel subunit; Cerebellum; Immunohistochemistry; Neuronal NO synthase (nNOS) knock-out (-/-) mice; Nitric oxide (NO); neuronal nitric oxide synthase; receptor subunit; voltage gated calcium channel; animal experiment; animal tissue; apoptosis; article; cerebellum cortex; cerebellum nucleus; controlled study; down regulation; immunohistochemistry; immunoreactivity; knockout mouse; male; mouse; nerve cell differentiation; nerve cell plasticity; neuromodulation; nonhuman; priority journal; protein expression; Purkinje cell; Animals; Calcium Channels; Cerebellum; Gene Expression Regulation; Immunohistochemistry; Male; Mice; Mice, Inbred C57BL; Mice, Knockout; Neurons; Nitric Oxide Synthase; Nitric Oxide Synthase Type I; Protein Subunits

제목: Immunohistochemical study of the distribution of neuronal voltage-gated calcium channels in the nNOS knock-out mouse cerebellum

저자: Kim, M.J.; Chung, Y.H.; Joo, K.M.; Oh, G.T.; Kim, J.; Lee, B.; Cha, C.I.

DOI: 10.1016/j.neulet.2004.07.047

발행일: 2004-10-07

유형: Article

저널명: Neuroscience Letters

권: 369

호: 1

페이지: 39 ~ 43

언어: ENG

발행국가: 아일랜드

분량: 5 페이지

ISSN: E 1872-7972
P 0304-3940