Aureimonas fodinaquatilissp. nov., isolated from coal mine wastewater

초록

A Gram stain-negative, aerobic, non-motile, short, rod-shaped bacterial strain CAU 1482(T)was isolated from coal mine wastewater in Hongcheon, Korea. It grew well at 30 degrees C, pH 8.5, 2% NaCl (w/v). 16S rRNA-based phylogeny indicated that CAU 1482(T)forms a distinct lineage withinAureimonaswith high similarity toAureimonas frigidaquaeCW5(T)(98.2%),Aureimonas altamirensisS21B(T)(98.0%), andAureimonas glacieiB5-2(T)(96.3%). The predominant cellular fatty acids were C(18:1)2-OH, C-16:0, C-18:1 omega 7c, and/or C-18:1 omega 6c(summed feature 8), with Q-10 as the major isoprenoid quinone. The polar lipid profile comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, two unidentified aminolipids, and three unidentified lipids. The 3.9-Mb genome included 8 contigs and 3599 protein-coding genes with a 56.7 mol% G + C content. Average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between strain CAU 1482(T)and closely related strains ofA. frigidaquaeCW5(T)andA. altamirensisS21B(T)were 72.2-72.4% and 18.7-18.8%, respectively. These phenotypic, chemotaxonomic, and phylogenetic data support CAU 1482(T)as a novelAureimonasspecies, for which the nameAureimonas fodinaquatilissp. nov. is proposed. The type strain is CAU 1482(T)(= KCTC 62995(T) = NBRC 113692(T)).

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