Paenibacillus konkukensis sp nov., isolated from animal feed

초록

A Gram-stain-positive, oxidase-and catalase-positive, aerobic, rod-shaped bacterium, designated strain SK-3146(T), was isolated from animal feed. Phylogenetic analysis, based on 16S rRNA gene sequence comparisons, revealed that the strain formed a distinct lineage within the genus Paenibacillus that was closely related to Paenibacillus yunnanensis JCM 30953(T) (98.6 %), Paenibacillus vulneris CCUG 53270(T) (98.0 %) and Paenibacillus chinjuensis DSM 15045(T) (96.9 %). Cells were nonmotile, endospore-forming and formed milky colonies on NA and R2A agar media. Growth of strain SK-3146(T) occurred at temperatures of 18-45 degrees C, at pH 6.0-9.5 and between 0.5-3.0% NaCl (w/v). The major menaquinone was MK-7, with lesser amounts of MK-6 present. The cell wall peptidoglycan of strain SK-3146(T) contained meso-diaminopimelic acid. The major fatty acids were anteiso-C-15 : 0 and iso-C-16 : 0. The major polar lipids were diphosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content was 53.8 mol% and the DNA-DNA hybridization relatedness values between strain SK-3146(T) and P. yunnanensis JCM 30953(T) and P. vulneris CCUG 53270(T) were 26.13 +/- 0.8% and 38.7 +/- 0.6 %, respectively. The phenotypic, phylogenetic and chemotaxonomic results indicate that strain SK-3146(T) represents a novel species of the genus Paenibacillus, for which the name Paenibacillus konkukensis sp. nov. is proposed. The type strain is SK-3146(T) (=KACC 18876(T) = LMG 29568(T)).

키워드