Calcium Phosphate Cement-Multi-Walled Carbon Nanotube Hybrid Material (CPC-MWCNT Hybrid) Enhances Osteogenic Differentiation

초록

Calcium phosphate cements (CPCs) have been widely used as bone substitute materials in medical and dental fields. Carbon nanotubes (CNTs) are promising materials as substrates for tissue engineering or for the use as implant materials. With a purpose to evaluate the potential of CPC-CNT based materials as bone grafting materials, we synthesized calcium phosphate cement-multi-walled carbon nanotube hybrid (CPC-MWCNT hybrid) and investigated the effect of CNT on enhancing the bioactivity of osteoblasts. The results were evaluated by cell proliferation and differentiation of MC3T3-E1 cells. No significant difference between CPC and CPC-MWCNT hybrid was found with respect to the proliferation of MC3T3-E1 cells. However in contrast, we found that the differentiation of MC3T3-E1 was significantly enhanced in the presence of MWCNT which is demonstrated by ALP activity analysis and RT-PCR. The results of the current study indicate that CPC-MWCNT hybrid which promotes the osteogenic differentiation of osteoblasts could serve well as bone repairing graft material.

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