상세 보기
- Lee, Chae Eun;
- Lee, Jeong Hwan;
- Chung, Hyun Joo;
- Lim, Sohee;
- Lee, Da Won;
- ... Kim, Jin Wook;
- ... Hong, Soon Auck;
- ... Hong, Min Eui;
- ... Kim, Joo Young;
- ... Lee, Hye Jun;
- ... Ko, Kisung;
- ... Myung, Soon Chul;
- 외 1명
WEB OF SCIENCE
3SCOPUS
3초록
Biosimilars produced in plant systems encompass a range of molecules, including antibodies, vaccines, enzymes, receptors, and other therapeutic molecules. In the present study, we aimed to express the immune checkpoint-inhibitor anti-CTLA-4 antibody, Ipilimumab, in Nicotiana tabacum plant. We generated transgenic tobacco plants using Agrobacterium-mediated transformation and purified an anti-CTLA-4 monoclonal antibody (mAb(P) CTLA-4) using protein A affinity chromatography. This purified mAb(P) CTLA-4 showed specific binding activity to CTLA-4-positive human tonsil tissues, as confirmed by immunoblot, indirect ELISA, and immunohistochemistry analyses. Our study confirmed that mAb(P) CTLA-4 effectively targeted CTLA-4 in human tonsil tissues. These findings suggest that our plant-derived anti-CTLA-4 antibody retains its original functionality, underscoring the promising potential of plant-based therapeutic expression systems for producing high-value medicinal biosimilars.
키워드
- 제목
- Expression and in vitro function of anti-CTLA-4 human antibody expressed in plants
- 저자
- Lee, Chae Eun; Lee, Jeong Hwan; Chung, Hyun Joo; Lim, Sohee; Lee, Da Won; Lim, Jong Seok; Kim, Jin Wook; Hong, Soon Auck; Hong, Min Eui; Kim, Joo Young; Lee, Hye Jun; Ko, Kisung; Myung, Soon Chul
- 발행일
- 2025-04
- 유형
- Article
- 권
- 19
- 호
- 2
- 페이지
- 197 ~ 204