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Recent advances in genome engineering by CRISPR technology
- Lee, Youngsik;
- Oh, Yeounsun;
- Lee, Seung Hwan
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6Citations
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12초록
Due to the development of CRISPR technology, the era of effective editing of target genes has arrived. However, the offtarget problem that occurs when recognizing target DNA due to the inherent nature of CRISPR components remains the biggest task to be overcome in the future. In this review, the principle of inducing such unintended off-target editing is analyzed from the structural aspect of CRISPR, and the methodology that has been developed to reduce off-target editing until now is summarized. [BMB Reports 2024; 57(1): 12-18].
키워드
CRISPR system; Gene editing; Specificity; RNA-GUIDED ENDONUCLEASE; TARGET DNA RECOGNITION; R-LOOP COMPLEX; STRUCTURAL BASIS; UNBIASED DETECTION; CAS9; CLEAVAGE; CPF1; NUCLEASES; SPECIFICITIES
- 제목
- Recent advances in genome engineering by CRISPR technology
- 저자
- Lee, Youngsik; Oh, Yeounsun; Lee, Seung Hwan
- 발행일
- 2024-01
- 유형
- Review
- 저널명
- BMB Reports
- 권
- 57
- 호
- 1
- 페이지
- 12 ~ 18