Production of inulooligosaccharides from inulin by a novel endoinulinase from Xanthomonas sp.

초록

A novel inulinolytic microorganism, Xanthomonas sp. produced an endoinulinase, to be used for inulooligosaccharide (IOS) formation from inulin, at an activity of 11 units ml-1 (1.2 mg protein ml-1). The endoinulinase was optimally active at 45 °C and pH 6.0. Batchwise production of IOS was carried out by the partially purified endoinulinase with a maximum yield of about 86% on a total sugar basis with 10 g inulin l-1. The major IOS components were DP (degree of polymerization) 5 and 6 with trace amount of smaller oligosaccharides.

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