Caspases Activation in Ultraviolet B-induced Apoptosis of G361 Human Melanoma Cell line.

초록

Background : Ultraviolet B (UVB) irradiation can induce apoptosis of melanocytes and melanoma cells. However, mechanism of UVB-induced apoptosis of melanoma cells is not clarified yet. Objective : Our purpose was to study the molecular mechanism of UVB-induced apoptosis of melanoma cells. Methods : G361 lightly pigmented melanoma cells were analyzed for apoptotic mechanism by flow cytometry and western blotting. Results : G361 melanoma cells showed apoptotic features with gradual increment of UVB doses by MTT and flow cytometry. Western blotting disclosed activation of caspase-3 and poly (ADP-ribose) polymerase (PARP) after UVB irradiation. Conclusion : In this study, we showed that UVB-induced apoptosis of melanoma cells is mediated by PARP activation which is induced by caspase cascade.

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