Immunohistochemical analysis of the distribution and quantification of internalized Escherichia coli, Listeria monocytogenes, and murine norovirus in intact and damaged potatoes

초록

This study aimed to verify the distribution of Escherichia coli, Listeria monocytogenes, and murine norovirus (MNV) internalized in potatoes using immunochemical analyses and quantify the internalization of these internalized bacteria and viruses. Damaged and intact potatoes were inoculated with pathogens and stored under controlled conditions to assess the internalization process over 14 days. Histological observations revealed that E. coli, L. monocytogenes, and MNV could penetrate potato tissues through the periderm cells, apoplast, and xylem, particularly in damaged potatoes, causing browning, lignification, and biofilm formation. Real-time quantitative PCR confirmed the internalization of pathogens: E. coli, L. monocytogenes, and MNV were detected at levels 1 log genome copy higher in damaged potatoes, respectively. This study also demonstrated hyperplastic activity in the phellogen layer in response to bacteria and viruses. These findings underscore the importance of preventing physical damage to potatoes during cultivation and pre- and post-harvest processes to mitigate the risk of pathogen contamination, potentially reducing foodborne illnesses associated with potatoes. © 2024

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