다공성막이 혈액뇌관문 내피세포의 배양에 끼치는 영향

초록

The growth patterns of primary culture of bovine brain microvessel endothelial cells (BBMECs) were studied using electron microscopy when grown on 3.0μm and 0.4μm pore Transwell. The capillary fragments and isolated endothelial cells grew on collagen coated culture plate and Transwell membrane. The BBMECs grew only on the upper surface of membrane of 0.4μm. But BBMECs on 3.0μm pore membrane migrated through the pore and grew on the opposite side of the membrane. In summary, BBMECs isolated by enzyme digestion could migrate through 3.0μm pore membrane but not through 0.4μm pore membrane. So 0.4μm pore membrane instead of 3μm pore membrane should be used for drug transport experiment or transendothelial electrical resistance measurement.

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