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Screening and selection of production strains: Secretory protein expression and analysis in Hansenula polymorpha
- Yoo, Su Jin;
- Moon, Hye Yun;
- Kang, Hyun Ah
Citations
SCOPUS
7초록
The thermotolerant methylotrophic yeast Hansenula polymorpha has been used as a host for the high-level production of recombinant proteins from industrial enzymes to therapeutic proteins. Despite favorable characteristics of the H. polymorpha-based platform for application to heterologous gene expression, several problems and limitations, such as over-glycosylation and proteolytic degradation, can be encountered in the development of production strains for secretory proteins. Here, H. polymorpha genetic tools and host strains, developed for authentic processing and modification of secretory recombinant proteins, are introduced with the analytical protocols.
키워드
Glycosylation; Hansenula polymorpha; Multiple integration; Protein expression; Secretion; Surface display; epidermal growth factor; fungal protein; glucose oxidase; hygromycin B; parathyroid hormone; phleomycin; recombinant protein; secretory protein; sulfate adenylyltransferase; epitope; fungal protein; fusion protein; polysaccharide; fungal cell wall; gene expression; high throughput screening; nonhuman; Ogataea angusta; promoter region; protein analysis; protein expression; protein folding; protein modification; cell wall; chemistry; gene vector; genetic marker; genetic transformation; genetics; glycosylation; high throughput sequencing; metabolism; molecular biology; Pichia; procedures; protein engineering; transgenic microorganism; Cell Wall; Epitopes; Fungal Proteins; Genetic Markers; Genetic Vectors; Glycosylation; High-Throughput Nucleotide Sequencing; Microorganisms, Genetically-Modified; Molecular Biology; Pichia; Polysaccharides; Promoter Regions, Genetic; Protein Engineering; Recombinant Fusion Proteins; Transformation, Genetic
- 제목
- Screening and selection of production strains: Secretory protein expression and analysis in Hansenula polymorpha
- 저자
- Yoo, Su Jin; Moon, Hye Yun; Kang, Hyun Ah
- 발행일
- 2019-02
- 유형
- Book Chapter
- 권
- 1923
- 페이지
- 133 ~ 151