Nanofibrous Mineralized Electrospun Scaffold as a Substrate for Bone Tissue Regeneration

초록

Fibrous scaffolds that reconstruct the extracellular matrix (ECM) have been utilized for tissue regeneration demonstrated potential for guiding stem cell differentiation. Nanofibrous scaffolds fabricated by a unique electrospinning method enabled us to create tailored, functional scaffolds on-demand. Several patterned electrospun poly(epsilon-caprolactone) (PCL) scaffolds were prepared, and then utilized for creating a hybrid composite in which bone-like hydroxyapatite (b-HA) was deposited onto the unique electrospun scaffolds. The mineral deposits onto the patterned PCL scaffolds was confirmed by scanning electron microscope (SEM). When culturing human adipose-derived stem cells (hASC) onto the different SBF-treated electrospun PCL scaffolds, it was found that the hybrid composite can support hASC differentiated into osteoblasts under osteogenic differentiation conditions. Image analysis and alamar blue assay indicated a significant increase of hASC adhesion and proliferation on the SBF-treated PCL scaffolds. Subsequent analysis of osteogenic potential by via gene expression analysis and alkaline phosphatase (ALP) activity also demonstrated that the SBF-treated electrospun PCL made by the modified electrospinning process is more favorable for the osteogenic differentiation hASCs. Additionally, results of alizarin red S staining and ALP staining at days 7 and 14 showed improved deposition of mineralized matrix on the SBF-treated PCL. Therefore, this study indicates that the facile scaffold fabrication method described in this study is promising approach to prepare osteoconductive scaffold for bone tissue engineering.

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