ScholarWorks@중앙대학교

초록

Global warming has quietly spread viruses previously confined to tropical regions amid public indifference. Among them, the mosquito-borne zoonosis Zika virus caused severe complications, with new symptoms reported recently that can lead to human deaths. To recognize and address this potential risk, the present study introduced an electrochemical biosensor for rapid point-of-care detection of Zika virus. Miniaturization of the device and analysis of small samples of 10 mu L were achieved through the use of micro-sized round-type electrodes. To amplify the detection signal by improving conductivity, MXene (Ti3C2Tx) was applied to the electrode surface, and the sensing structure complexity was reduced by forming a sensing double layer through sequential functionalization of the truncated aptamers. The fabricated device was able to detect Zika envelope protein in the concentration range of 10 pM to 1 mu M within 10 min using alternating current electrochemical flow technology. Under the target diluted in deionized water (DIW) and 10 % human serum, the sensor showed excellent reproducibility and selectivity, and the detection limits were confirmed 6.26 pM and 3.82 pM, respectively. The developed biosensing platform provides electrochemical insights for detecting the Zika virus in clinical conditions. This can be applied as an attractive tool for constructing a patient-convenient and economical system for target virus detection. In addition, rapid analysis based on improved device performance compared to previous devices suggests the practical applicability of point-of-care diagnosis.

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