Effect of Miscanthus sinensis var. purpurascens Flower Extract on Proliferation and Molecular Regulation in Human Dermal Papilla Cells and Stressed C57BL/6 Mice

초록

Objective: To investigate the hair growth-promoting effect of Miscanthus sinensis var. purpurascens (MSP) flower extracton on in vitro and in vivo models. Methods: MSP flower extract was extracted in 99.9% methanol and applied to examine the proliferation of human dermal papilla cells (hDPCs) in vitro at the dose of 3.92-62.50 mu g/mL and hair growth of C57BL/6 mice in vivo at the dose of 1000 mu g/mL. The expression of transforming growth factor beta 1 (TGF-beta 1), hepatocyte growth factor (HGF), beta-catenin, substance P was measured by relative quantitative realtime polymerase chain reaction. Histopathological and immunohistochemical analysis were performed. Results: MSP (7.81 mu g/mL) down-regulated TGF-beta 1 and up-regulated HGF and beta-catenin in hDPCs (P<0.01). MSP (1000 mu g/mL)-treated mice showed the earlier transition of hair follicles from the telogen to the anagen phase. The number of mast cells was lower in the MSP-treated mice than in other groups (P<0.05 vs. NCS group). Substance P and TGF-beta 1 were expressed in hair follicles and skin of the MSP group lower than that in negative control. Stem cell factor in hair follicles was up-regulated in the MSP-treated mice (P<0.01). Conclusion: The MSP flower extract may have hair growth-promotion activities.

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