형질전환식물체 세대 간 항암단일항체 발현안정성

초록

Recent plant biotechnology and advanced molecular immunology have established plant biopharming system as an alternative way to produce immunotherapeutic monoclonal antibodies. This plant production system is considered to have several advantages in safety and economical issues compared to other production systems. In this study, we confirmed stable gene insertion and expression of anti-cancer monoclonal antibody (mAb CO17-1A) in transgenic plants through subsequent generation. T-3 and T-4 generation of transgenic seeds were obtained by self-fertilization of homozygous transgenic line of T-2 and T-3 generation, respectively. T3 and T-4 seedlings showed 100% of germination and survival rate in MS media containing kanamycin whereas non-transgenic seedlings did not survive. PCR analysis showed that stable gene insertion of heavy and light chains of mAb CO17-1A in T-3 and T-4 transgenic plants. Immunoblot with densitometric analysis confirmed stable expression of mAb CO17-1A in both T-3 and T-4 transgenic plants. These results suggested that transgenes and expression of anti-cancer mAb CO17-1A are stably transferred and expressed in transgenic plants through subsequent generation by self fertilization, which is applicable to generate seed bank of homozygous transgenic line.

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