Kainic Acid에 의한 흰쥐 해마 신경원 가지돌기의 독성변화에 대한 광학 및 전자현미경 연구

초록

The dendrotoxic effect in hippocampal regions was studied light and electron microscopically by intraperitonial injection of 1mg and 2mg of kainic acid into rat. The results obtained were as follows. 1) Light microscopic change of swelling was observed in all the 2mg kainic acid group (1-24 hours) and not in the 1mg kainic acid group (1-24 hours). The prominent laminar Pattern of swelling along pyramidal cell body layer and dendritic fields was prominent at 2-4 hours after kainic acid injection. 2) In ultrastructural study, the swelling was seen only in dendrites of pyramidal cells in both 1mg and 2mg groups. Dendrites with severe swelling could be identified by intact synapse with nerve terminals. The swelling in dendrite was the earlist change and not observed in other neuropil by injection of kainic acid. 3) The morphological change was prominent along the proximal but mild along distal basilar dendritic fields of CA3, 4. The change was mild in the distal dendritic field in CA3. The morphological change was prominent along the proximal and distal basilar dendritic fields of CA1, 2, but mild along the distal apical dendritic fields of CA1. No change was observable in dentate granule cell. 4) With the severity and the frequency of swelling change, the sensitivity of hippocampal fields to kainic acid could be put CA4, CA4, CA1, CA2 and dentate granule cell with decreasing degree in order.

키워드