인공피부 모델을 이용한 hGH의 효능연구

초록

In this study, the effects of hGH were analyzed by using living skin equivalents(LSEs) models. In monolayer culture of keratinocytes, hGH was not cytotoxic to cells in the range of 0.0005 similar to 0.5%. However, hGH inhibit keratinocytes proliferation in all tested concentrations. In fibroblasts, hGH was slightly toxic to fibroblasts at 0.5% concentration. However, hGH stimulate fibroblasts proliferation at the concentrations between 0.005 similar to 0.05%. These results showed that hGH is not cytotoxic to epidermal cells up to 0.5% concentration. But. it can be said that different pathways are mediated by hGH in keratinocytes and fibroblasts. Then, the effects of hGH were tested using LSE models. For this study, 0.05% concentration of hGH was used. It is thought that epidermis in LSE is hyperproliferative. Thus, parakeratotic epidermis is observed and granular layer is not well formed. To suppress the hyperproliferative potential of LSEs, high concentration of rh-EGF(10 ng/ml) was usually added in the preparation of LSEs. In this study, 0.05% hGH was added and epidermal thickness of hGH treated model looked slightly thinner than that of rh-EGF models. Interestingly, parakeratosis was not observed in both rh-EGF and hGH models. Thus. it can be said that rh-EGF and hGH can control the hyper-proliferation of keratinocytes in the reconstruction of SEs. In the reconstruction of epidermis, basement membrane formation is also very important. Results showed that alpha 6 integrin and B, integrin expression is elevated in hGH models. Furthermore, involucrin expression was evident at upper epidermis. However, expression of PCNA and p63 was not different compared to other models. Results suggest that hGH promote the formation of basement membrane and differentiation.

키워드