Flexivirga oryzae sp nov., isolated from soil of a rice paddy, and emended description of the genus Flexivirga Anzai et al. 2012

초록

A Gram-staining-positive, strictly aerobic bacterium, designated R1(T), was isolated from a rice paddy in South Korea. Cells were non-motile cocci showing oxidase-negative and catalase-positive activities. Growth of strain R1(T) was observed at 1037 degrees C (optimum, 30 degrees C) and pH 5.0-9.0 (optimum, pH 7.0). Strain R1(T) contained iso-C-16 : 0, summed feature 9 (comprising iso-C-17 : 1! 9c/10- methyl C-16 : 0), anteiso-C-17 : 0, iso-C-17 : 0 and anteiso-C-17 : 1! 9c as the major fatty acids and MK-8 (H-4) and MK-8 (H-6) as the isoprenoid quinones. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, three unidentified phospholipids, four unidentified aminolipids, one unidentified glycolipid and four unidentified lipids. The peptidoglycan type was A4 alpha with an L-Lys-L-Ser(2) D-Glu interpeptide bridge containing a Gly residue. The G+C content of the genomic DNA was 64.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain R1(T) was most closely related to the members of the genus Flexivirga, Flexivirga endophytica YIM 7505(T) (97.7 %), Flexivirga luteaTBS-100(T) (97.5 %) and Flexivirga alba ST13(T) (97.2 %). On the basis of phenotypic, chemotaxonomic and molecular properties, it is clear that strain R1(T) represents a novel species of the genus Flexivirga, for which the name Flexivirga oryzae sp. nov. is proposed. The type strain is R1(T) (= KACC 18597(T) = JCM 31060(T)). An emended description of the genus Flexivirga is also proposed.

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