ScholarWorks@중앙대학교

초록

Oxidative stress plays a central role in the development of chronic degenerative diseases, prompting growing interest in natural antioxidants, particularly phenolic compounds from early developmental plant tissues. This study investigated the chemical composition and antioxidant capacity of Acer tegmentosum sprouts at the cotyledon expansion stage using integrated metabolite profiling and targeted quantification approaches. A. tegmentosum sprout extracts (ASE) were characterized by liquid chromatography–tandem mass spectrometry (LC–MS/MS) for untargeted metabolite annotation and by high-performance liquid chromatography (HPLC) for the targeted quantification of selected phenolic acids and coumarins using authentic standards. Antioxidant activity was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging assays. LC–MS/MS analysis identified twelve phenolic compounds, including gallic acid, protocatechuic acid, scopoletin, and their derivatives. HPLC results confirmed gallic acid (5.54 mg/g extract) as the predominant phenolic constituent, and the overall composition indicated a phenolic acid-enriched profile. ASE showed notable DPPH and ABTS radical scavenging activities, with IC50 values of 376.40 and 311.00 μg/mL, respectively, although these activities were lower than those of ascorbic acid. Overall, these findings define the baseline chemical and antioxidant properties of ASE and identify analytically traceable marker compounds for standardization and functional material development. Further studies across different developmental stages and cultivation conditions are needed to verify generalizability and refine marker selection for quality control purposes.

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