Quantitative analysis of lignans in the fruits of Acanthopanax species by HPLC

초록

High performance liquid chromatography (HPLC) was used for the analysis of the lignans eleutheroside B and eleutheroside E in the fruits of Acanthopanax species. A reverse-phase system using a gradient of H2O and acetonitrile as the mobile phase was developed and detection was at 210 nm. The analysis was successfully carried out within 20 min. The content of eleutheroside B and eleutheroside E in Acanthopanax species was measured in the fruits of A. senticosus (0.58 and 1.66 mu g/mg, respectively), A. sessiliforus (1.15 and 8.49 mu g/mg, respectively), A. koreanum (2.16 and 1.80 mu g/mg, respectively), and A. divaricatus (1.06 and 7.08 mu g/mg, respectively).

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