Inhibition of FoxO1 acetylation by INHAT subunit SET/TAF-I beta induces p21 transcription

초록

Post-translational modification of forkhead family transcription factor, FoxO1, is an important regulatory mode for its diverse activities. FoxO1 is acetylated by HAT coactivators and its transcriptional activity is decreased via reduced DNA binding affinity. Here, we report that SET/TAF-I beta inhibited p300-mediated FoxO1 acetylation in an INHAT domain-dependent manner. SET/TAF-I beta interacted with FoxO1 and activated transcription of FoxO1 target gene, p21. Moreover, SET/TAF-I beta inhibited acetylation of FoxO1 and increased p21 transcription induced by oxidative stress. Our results suggest that SET/TAF-I beta inhibits FoxO1 acetylation and activates its transcriptional activity toward p21. Structured summary of protein interactions: FoxO1 physically interacts with SET/TAF-I beta by pull down (1, 2) FoxO1 physically interacts with SET/TAF-I beta by anti tag coimmunoprecipitation ( View interaction) SET/TAF-I beta physically interacts with FoxO1 by anti bait coip (1, 2) (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

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