Novel simple sequence length polymorphic (SSLP) markers for positional cloning in Arabidopsis thaliana

초록

T-DNA-based tagging technology facilitated isolation of biologically important genes from the Arabidopsis genome. However, the technology frequently results in untagged mutants that are free of T-DNA but contain mutations due to deletions of varying size of genomic DNA. To clone a gene from the T-DNA or ethylmethanesulfonate (EMS)-mutagenized point mutant populations, map-based cloning method should be exercised. Arabidopsis genome database contains a great number of genetic markers, but easy-to-use PCR-based markers are only scarcely distributed over the genome especially for other ecotypes than Columbia and Landsberg. For this reason, we have developed 23 novel simple sequence length polymorphism (SSLP) markers in the cloning process of an EMS-induced point mutation of the gulliver2 gene. We examined polymorphisms of these novel markers from 4 different ecotypes, Columbia, Wassilevskija-2, Landsberg erecta, and Enkheim-2. These markers will be useful for genotyping and map-based cloning of a gene in Arabidopsis. © The Genetics Society of Korea.

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