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N-acetylcysteine enhances neuronal differentiation of P19 embryonic stem cells via Akt and N-cadherin activation
- Noh, Y.-H.;
- Cho, H.-S.;
- Kim, D.-H.;
- Kim, O.H.;
- Park, J.;
- ... Chung, Yoon Hee;
- ... Kim, Kyung Yong;
- 외 7명
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2초록
We examined whether N-acetylcysteine (NAC) enhanced embryonic body (EB) formation and neuronal differentiation in terms of EB formation, neuronal marker (microtubule-associated protein 2; MAP-2) expression, and neuron maturation using P19 embryonic stem cells. The size and numbers of EBs were greatly increased, together with the up-regulated N-cadherin expression. Also, MAP-2 expression and neurite outgrowth were much increased with activation of serine/threonine protein kinase (Akt) and blocked by addition of an Akt inhibitor (LY294002). Our results suggested that NAC increased EB formation by up-regulating the N-cadherin expression. Furthermore, NAC-enhanced neuronal differentiation was mediated by activation of Akt.
키워드
Akt; N-cadherin; N-acetylcysteine; P19 embryonic stem cell; differentiation; CARCINOMA-CELLS; EXPRESSION; PATHWAY; PROTEIN; BRAIN
- 제목
- N-acetylcysteine enhances neuronal differentiation of P19 embryonic stem cells via Akt and N-cadherin activation
- 저자
- Noh, Y.-H.; Cho, H.-S.; Kim, D.-H.; Kim, O.H.; Park, J.; Lee, S.-A.; Yang, Hoon-Shik; Sohn, Dong-Suep; Kim, W.; Kim, D.; Chung, Yoon Hee; Kim, Kyung Yong; Kim, S.S.; Lee, W.B.
- 발행일
- 2012-09
- 유형
- Article
- 권
- 46
- 호
- 5
- 페이지
- 664 ~ 669