Detection and Quantification of mRNA in Mammalian Spermatozoa

Pang, Won-Ki; Pang, Myung-Geol

doi:10.1007/978-1-0716-4406-5_30

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Detection and Quantification of mRNA in Mammalian Spermatozoa

Pang, Won-Ki;
Pang, Myung-Geol

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초록

Spermatozoa have unique RNA biology compared to the other somatic cells. Therefore, sample collection to final quantification requires an optimized method. Here, we described proper processing methods to quantify sperm mRNA. © 2025. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

키워드

Guanidine-isothiocyanate lysis buffer; RT-qPCR; Silica-based column; Spermatozoa; messenger RNA

제목: Detection and Quantification of mRNA in Mammalian Spermatozoa

저자: Pang, Won-Ki; Pang, Myung-Geol

DOI: 10.1007/978-1-0716-4406-5_30

발행일: 2025

유형: Journal Article

저널명: Methods in molecular biology (Clifton, N.J.)

권: 2897

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