Blood Transcriptomic Changes in Heat-Stressed Finishing Pig

초록

Pork consumption continues to increase worldwide, but with the acceleration of global heating, pigs are affected by heat stress that leads to reduce animal productivity. Although solutions of heat stress environments have been reported in various fields, research on transcriptome-based physiological changes is not enough. In this study, differentially expressed gene (DEG) profiling and gene ontology (GO) terms were identified through RNA-Seq analysis using whole blood of pig, categorized by the presence or absence of heat stress. As a result of DEG profiling, 354 genes were differentially expressed in week 1, and 99 genes were differentially expressed in week 2. In week 1, the TLR9 included in Toll-like receptor 9 signaling pathway was identified as a biological process (BP). Also, NOTCH1 was identified, which has an immunosuppressive effect by promoting and increasing TGF-β signaling. And in week 2, ALAS2 and FECH were identified as significant genes commonly involved in BP and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway. As a result of functional enrichment analysis, candidate genes specifically expressed in pig heat stress environment were identified. This study provides insight into comprehensive understanding of heat-stressed pigs from a transcriptomic perspective.

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