Kaistia algarum sp nov., isolated from a freshwater green alga Paulinella chromatophora

초록

A Gram-negative, strictly aerobic, non-motile and short rod-or coccus-shaped bacterium, designated strain LYH11(T), was isolated from a freshwater green alga Paulinella chromatophora. The strain grew at 5-37 degrees C (optimum, 30 degrees C) and pH 6-9 (pH 7) and in the presence of 0-1% (w/v) NaCl (optimum, 0 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain LYH11(T) clearly belonged to the genus Kaistia of the family Rhizobiaceae. Strain LYH11(T) shared the highest 16S rRNA gene sequence similarity to Kaistia soli 5YN9-8(T) (98.3 %), Kaistia terrae 5YN3-3(T) (98.2 %), Kaistia geumhonensis B1-1(T) (97.8 %), Kaistia defluvii B6-12(T) (97.4 %) and Kaistia granuli Ko04(T) (97.2 %). The average nucleotide identity and in silico DNA-DNA hybridization values between strain LYH11(T) and K. soli 5YN9-8(T), the closest Kaistia type strain, were 77.3 and 21.1 %, respectively. Major cellular fatty acids of strain LYH11(T) were cyclo-C-19 : 0 omega 8c, summed feature 8 (C-18 : 1 omega 7c and/or C-18 : 1 omega 6c), iso-C-10 : 0, iso-C-17 : 0 3-OH, iso-C-17 : 1 omega 5c and C-18 : 0. Strain LYH11(T) contained phosphatidylglycerol, phosphatidylethanolamine, an unidentified phosphoaminolipid, an unidentified aminolipid, three unidentified phospholipids and five unidentified lipids as polar lipids. Ubiquinone-10 was the major respiratory quinone. The genomic DNA G+C content was 64.5 mol%. Based on the genotypic, chemotaxonomic and phenotypic analyses, strain LYH11(T) represents a novel species of the genus Kaistia, for which the name Kaistia algarum sp. nov. is proposed. The type strain is LYH11(T) (= KACC 19096(T) = JCM 31803(T)).

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