ScholarWorks@중앙대학교

초록

CD200 is a transmembrane glycoprotein, which has an anti-inflammatory effect through interaction with CD200 receptor 1 and inhibits the NF-kappa B pathway associated with loss of barrier function. In this study, CD200 was fused with the Fc domain of IgG to generate recombinant human CD200-Fc. The CD200-Fc was produced in the transgenic Nicotiana tabacum plant by Agrobacterium-mediated transformation. Genomic DNA PCR and RT-PCR analyses verified the successful insertion of transgene and transcription of CD200-Fc mRNA in the leaves of the plant transformants, respectively. Immunoblot analysis confirmed expression of CD200-Fc in the transgenic plant. The plant-derived CD200-Fc (CD200-FcP) was successfully purified by affinity chromatography. The qRT-PCR and immunoblot analyses confirmed that mRNA and protein levels of Zonula occludens-1, a tight junction protein for barrier protection, increased in TCCSUP bladder epithelial cells treated with CD200-FcP. These results suggest that CD200-Fc expressed in a plant system could exert beneficial effects on diseases with barrier dysfunction such as interstitial cystitis by regulating Zonula occludens-1 expression in bladder epithelial cells.

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