소나무 잎과 가지의 정유와 그 성분들의 항산화 및 항노화 작용

초록

To investigate effects of pine essential oil isolated by steam distillation from Pinus densiflora needles (PN) and twigs (PT) on anti-oxidant and anti-aging activities, the experiments were carried out to determine anti-oxidant and antiaging activities on DPPH radical scavenging activity, NBT/xanthine oxidase-superoxide scavenging activity,silica-induced intracellular H2O2 and hydroxyl radical generation in RAW264.7 cells, hyaluronidase and elastase activities in vitro. Essential oil of PN and PT were contained 0.225 and 0.176 (w/v) %, respectively. PN was contained with 30 kinds of essential oil and its major constituent is α-pinene (21.5%). Further PT was contained with 40 kinds of essential oil and its major constituent is β-pinene (22.4%) in GC/MS assay. Other essential oils of PN were β-pinene, camphene, myrcene, limonene, terpinolene,α-terpineol, bornyl acetate, α-copaene, caryophellene and humulene and PT were α-pinene, camphene, phellandrene, limonene, terpinolene, α-terpineol, bornyl acetate,α-copaene, caryophellene and humulene. The essential oil of PT have more active than that of PN in anti-oxidant activity which has significant DPPH radical and superoxide scavenging activity, and significant inhibitory activities on silica-induced intracellular H2O2 and hydroxyl radical generation, as well as, significantly inhibited elastase and hyaluronidase activities. Further, phellandrene, myrcene and α-pinene have DPPH radical and superoxide scavenging activities, β-pinene, terpinolene, myrcene and phellandrene inhibited silica-induced intracellular H2O2 and hydroxyl radical generations. And also phellandrene and β-pinene inhibited hyaluronidase and elastase activities. In conclusion, the essential oils isolated from PN and PT have anti-oxidant and anti-aging activities.

키워드