Blue Emissive Vanadium Nanoclusters-A Robust Nanosensor for the Sensitive Quantification of Selenite as an Oxidative Stress Biomarker

초록

A new strategy was developed by doping histidine (His) in bovine serum albumin (BSA) capped vanadium nanoclusters (VNCs) to enable the selective sensing of selenite as an oxidative stress biomarker. The as-fabricated BSA-His-VNCs possess good water solubility, ultra-small size, and good fluorescent properties. The as-synthesized BSA-His-VNCs displayed an emission peak at 410 nm when excited at 330 nm. Upon the introduction of selenite, the emission intensity of BSA-His-VNCs at 410 nm was significantly quenched (50%). The fluorescence lifetime of BSA-His-VNCs (1.38 ns) was reduced to 1.09 ns in the presence of selenite. Furthermore, BSA-His-VNCs demonstrate good selectivity for selenite over other similar structured oxygen-containing agents and metal ions. This method showed the limit of detection (LOD) of 0.50 mu M for selenite, respectively. The fluorescence turn-off sensing platform based on BSA-His-VNCs demonstrated superior sensing performance in assaying selenite in water samples, indicating the method has high potential for real-world uses.

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