Sphingobacterium humi sp nov., isolated from soil

초록

A Gram-stain-negative, facultatively aerobic bacterium, designated strain D1(T), was isolated from soil in South Korea. Cells of strain D1(T) were non-motile rods with oxidase- and catalase-positive activities. Growth was observed at 15-40 degrees C (optimum, 30-37 degrees C), at pH 5.5-9.0 (optimum, pH 7.0-8.0) and in the presence of 0.0-5.0% (w/v) NaCl (optimum, 0.0-1.0 %). The only respiratory quinone detected was menaquinone 7 (MK-7), and iso-C-15:0, iso-C-17:0 3-OH and summed feature 3 (comprising C-16:1 omega 7c/C-16:1 omega 6c) were identified as the major fatty acids. Phosphatidylethanolamine was the major polar lipid, and two unidentified glycophospholipids and four unidentified lipids were also detected as minor polar lipids. Sphingolipids, a typical chemotaxonomic feature of the genus Sphingobacterium, were detected. The G+C content of the genomic DNA was 43.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain D1(T) formed a phyletic lineage with Sphingobacterium hotanense XH4(T) within the genus Sphingobacterium. Strain D1(T) was most closely related to S. hotanense XH4(T) (98.1% 16S rRNA gene sequence similarity) and Sphingobacterium cellulitidis R-53603T (97.2 %), and the DNA-DNA relatedness level between strain D1(T) and the type strain of S. cellulitidis was 43.1 +/- 0.7 %. Based on the phenotypic, chemotaxonomic and molecular features, strain D1(T) clearly represents a novel species of the genus Sphingobacterium, for which the name Sphingobacterium humi sp. nov. is proposed. The type strain is D1(T) (=KACC 18595(T)=JCM 31225(T)).

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