Parahaliea aestuarii sp nov., isolated from the Asan Bay estuary

초록

A Gram-stain-negative, strictly aerobic and moderate halotolerant bacterial strain, designated S2-26(T), was isolated from sediment of the Asan Bay estuary in South Korea. Cells were motile rods with two polar flagella showing oxidase and catalase activities. Growth of S2-26(T) was observed at 15-45 degrees C (optimum, 25 degrees C) and pH 5.5-10.0 (optimum, pH 7.0-8.5) and in the presence of 0-8.0% (w/v) NaCl (optimum, 2.0 %). S2-26(T) contained C-17:1 omega 8c, summed feature 8 (comprising C-18:1 omega 7c and/or C-18:1 omega 6c), summed feature 3 (comprising C-16:1 omega 7c/C-16:1 omega 6c) and C-17:0 as the major fatty acids and ubiquinone-8 as the sole isoprenoid quinone. The polar lipids of S2-26(T) consisted of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, an unknown phospholipid, an unknown aminolipid, an unknown glycolipid and an unknown lipid. The G+C content of the genomic DNA was 62.2 mol%. The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that S2-26(T) formed a tight phylogenetic lineage with Parahaliea mediterranea 7SM29(T) with a 100% bootstrap value. S2-26(T) was most closely related to the type strain of Parahaliea mediterranea, with a 97.8% 16S rRNA gene sequence similarity, and its DNA-DNA relatedness level was 45.2 +/- 2.2 %. On the basis of phenotypic, chemotaxonomic and molecular properties, it is clear that S2-26(T) represents a novel species of the genus Parahaliea, for which the name Parahaliea aestuarii sp. nov. is proposed. The type strain is S2-26(T) (=KACC 18801(T) =JCM 31547(T)).

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