Chryseobacterium rigui sp nov., isolated from an estuarine wetland

초록

A bacterium, designated strain CJ16(T), was isolated from the estuarine wetland of the Han River. Cells of the isolate were yellow-pigmented, Gram-stain-negative, non-motile and rod-shaped. Growth of strain CJ16(T) was observed in TSB at 5-37 degrees C (optimum 30 degrees C), at pH 5.0-9.0 (optimum pH 6.0) and with 0-3% (w/v) NaCl (optimum 0%). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CJ16(T) was most closely related to Chryseobacterium hagamense KCTC 22545(T) (97.20/o 16S rRNA gene sequence similarity). Genomic relatedness based on DNA-DNA hybridization between strain CJ16(T) and C. hagamense KCTC 22545(T) was 23% (strain CJ16(T) as probe) and 19% (strain KCTC 22545(T) as probe). Chemotaxonomic analysis revealed that strain CJ16(T) possessed MK-6 as the major isoprenoid quinone and sym-homospermidine as the predominant polyamine. The predominant fatty acids were iso-C-15:0 (26.9%), iso-C-17:0 3-OH (16.8%) and summed feature 9 (comprising C-16:0 10-methyl and/or iso-C-17:1 omega 9c; 10.5%). The DNA G+C content of strain CJ16(T) was 37.9 mol%. Based on phenotypic, genotypic and phylogenetic studies, strain CJ16(T) represents a novel species of the genus Chryseobacterium, for which the name Chryseobacterium rigui sp. nov. is proposed. The type strain is CJ16(T) (=KACC 16560(T) =JCM 18078(T)).

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