Phenolic compounds from barks of Actinidia arguta planchon growing in Korea and its anti-oxidative and nitric oxide production inhibitory activities

초록

Phytochemical examination of the barks of Actinidia arguta led to the isolation of five flavonoids. Structures of compounds were elucidated as catechin (1), (-)-epicatechin (2), quercetin (3), quercetin-3-O-β-D- glucopyranoside (4), quercetin-3-O-β-D-galactopyranoside (5) by comparison with previously reported spectral evidences. To investigate the anti-oxidative effect and nitric oxide (NO) production inhibitory activity of these compounds, DPPH radical scavenging activity and nitric oxide production inhibitory activity in IFN-γ, LPS stimulated RAW 264.7 cell were examined. The IC 50s were determinied as follows: 1 IC50 = 26.61 μg/ml, 2 IC50 = 25.30 μg/ml, 3 IC50 = 20.41 μg/ml, 4 IC50 = 18.23 μg/ml, 5 IC50 = 30.46 μg/ ml, 6 IC 50 = 28.03 μg/ml and 7 IC50 = 27.24 μg/ml. These NO production inhibitory effects were significantly different compared with the positive control, L-NMMA (IC50 = 20.77 μg/ml), respectively. Compound 1 (IC50 = 6.19 μg/ml), 2 (IC50 = 8.98 μg/ml), 3 (IC50 = 7.30 μg/ml) and 4 (IC50 = 7.64 μg/ml) also showed potent antioxidative activities similar level to ascorbic acid (IC50 = 9.22 μg/ml). These results suggest that barks of A. arguta have a potent anti-oxidative and anti-inflammatory activity.

키워드