Supercritical CO2 for Efficient Extraction of Polymethoxyflavones in Kaempferia parviflora

초록

To ensure polymethoxyflavone (PMF) extract from krachaidum, Kaempferia parviflora rhizome, supercritical CO2 extraction was attempted as an efficient and nontoxic method. Extraction yields and composition of PMFs in the supercritical fluid extracts (SFEs) were obtained under the different extraction conditions of pressure and temperature. Nine PMFs, including 5,7-dimethoxyflavone (1), 5,7,4'-trimethoxyflavone (2), 5,7,3',4'-tetramethoxyflavone (3), 5-hydroxy-7-methoxyflavone (4), 5-hydroxy-3,7-dimethoxyflavone (5), 5-hydroxy-3,7,3',4'-tetramethoxyflavone (6), 3,5,7-trimethoxyflavone (7), 3,5,7,4'-tetramethoxyflavone (8) and 3,5,7,3',4'-pentamethoxyflavone (9), were isolated by vacuum liquid chromatography and characterized using various spectroscopic methods. The extraction yield by supercritical CO2 extraction was highest at 60 degrees C and 300 bar, and the content of PMFs reached up to 85% in the SFEs.

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