ScholarWorks@중앙대학교

초록

This study investigated the anti-inflammatory effects of a short-chain fatty acid (SCFA)-enriched fermented blueberry supernatant (BBS) and its key component, propionic acid (PropA), in both in vitro and in vivo models of colitis. High-performance liquid chromatography confirmed the presence of PropA in BBS at a concentration of 1.86 mg/mL. In LPS-stimulated RAW 264.7 macrophages, BBS significantly reduced nitric oxide, monocyte chemoattractant protein-1, and interleukin-6 production in a dose-dependent manner. Western blotting revealed that BBS suppressed the expression of inducible nitric oxide synthase, COX-2, and NF-κB. In a DSS-induced colitis mouse model, BBS administration significantly improved disease indicators, including body weight loss, colon shortening, and spleen enlargement. Moreover, BBS restored serum IgG and colonic IgA levels and reduced systemic and local inflammatory mediators. These findings highlight the potential of SCFA-enriched fermented blueberry as a functional postbiotic food capable of modulating inflammatory responses through NF-κB and COX-2 signaling pathways in colitis.

키워드