Hanseniaspora thailandica BC9 beta-Glucosidase for the Production of beta-D-Hexyl Glucoside

초록

For biotechnological production of high-valued beta-D-hexyl glucoside, the catalytic properties of Hanseniaspora thailandica BC9 beta-glucosidase purified from the periplasmic fraction were studied, and the transglycosylation activity for the production of beta-D-hexyl glucoside was optimized. The constitutive BC9 beta-glucosidase exhibited maximum specific activity at pH 6.0 and 40 degrees C, and the activity of BC9 beta-glucosidase was not significantly inhibited by various metal ions. BC9 beta-glucosidase did not show a significant activity of cellobiose hydrolysis, but the activity was rather enhanced in the presence of sucrose and medium-chain alcohols. BC9 beta-glucosidase exhibited enhanced production of beta-D-hexyl glucoside in the presence of DMSO, and 62% of beta-D-hexyl glucoside conversion was recorded in 4 h in the presence of 5% 1-hexanol and 15% DMSO.

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