국산 종계 개발을 위한 토종 계통들의 스트레스 반응 정도 분석

초록

We compared the stress responses of 12 domestic purebred chicken strains that have been bred at the National Institute of Animal Science, RDA, Korea since 1980. As a physiological marker of the stress response, the expression levels of heat shock protein (HSP)-70, HSP-90α, HSP-90β, and hydroxyl-3-methyl-glutaryl coenzyme A reductase (HMGCR) genes and telomere length were measured by quantitative real-time polymerase chain reaction using the lymphocytes of 1,101 chickens. There were significant differences in the HSP-70, HSP-90α and HMGCR expressions and telomere lengths between the strains. There were also significant differences in the HSP-90α, HSP-90β and HMGCR expressions between the male and female chickens. Chickens in different age groups exhibited different levels of HSP-70 and HSP-90α expression and telomere length. The HSP expression level results suggested that the R, L and Y strains were highly resistant to stress, whereas the S, O and W strains were susceptible to stress. Although significant differences in some of HSP gene expression levels existed the between genders, the HSP expression results varied across the different strains, occasionally in opposite directions, and there might have been interactions between the strains and genders. Therefore, it was concluded that there were no differences in the stress responses of the male and female chickens. Moreover, despite the significant differences in some of the HSP expression levels, it was concluded that there were no differences in the stress responses according to age due to the inconsistent trends in the HSP markers.

키워드