ScholarWorks@중앙대학교

초록

Surface-enhanced Raman scattering (SERS) is a technique that enables the detection of very low concentrations of substances by significantly enhancing the Raman scattering signal of molecules adsorbed on nanostructured metal surfaces. Although multi well array SERS platforms have since been developed to hold large quantities of samples, conventional methods involving single-point measurements using a single probe and manual stage movement lead to slow well mapping that takes tens of minutes to hours, limiting their application for high throughput screening (HTS) purposes. In this work, we present a method for fast Raman spectroscopy in multi well array SERS using an automated single array Raman probe system based on optical switch Raman spectroscopy (OSRS). In the process of building the system, the size of the Raman probe array was reduced by about 44% to match the well size, and Raman data for 96 samples were automatically measured and acquired with a single click by interlocking hardware (auto stage, optical switch) and software (LabVIEW, MATLAB) in a measurement method using a manual stage. To evaluate the system performance, a vitamin C measurement experiment was conducted under the most similar conditions possible to compare the commercialized Raman microscope (HEDA) and the previous OSRS. In duplicate, three compounds, Malachite green (MG), 4-Aminothiophenol (4-ATP), and 4-Mercaptobenzoic acid (4-MBA), were randomly placed in 96 wells to perform automated measurements. The total measurement time was 5 minutes and 16 seconds. The spectra of the SERS signals were expressed using normalized intensity and visualized by Raman mapping. © 2025 SPIE.

키워드