Capmatinib attenuates lipogenesis in 3T3-L1 adipocytes through an adenosine monophosphate-activated protein kinase-dependent pathway
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초록

Recently, there is a rapid increase in the incidence of obesity, a condition for which there are no effective therapeutic agents. Capmatinib (CAP), a novel mesenchymal-to-epithelial transition inhibitor, is reported to attenuate pro-inflammatory mediators and oxidative stress. In this study, the effects of CAP on lipogenesis in the adipocytes were examined. Treatment with CAP dose-dependently suppressed lipid accumulation in, and differentiation of, and increased lipolysis in, 3T3-L1 adipocytes. Additionally, CAP treatment augmented adenosine monophosphate-activated protein kinase (AMPK) phosphorylation and FNDC5 expression in the adipocytes. Transfection with si-AMPK or si-FNDC5 mitigated the CAP-induced suppression of lipogenesis and enhanced lipolysis. Furthermore, transfection with si-FNDC5 mitigated the CAP-induced phosphorylation of AMPK. These results suggest that the anti-obesity effect of CAP is mediated through the irisin/AMPK pathway and that CAP is a novel therapeutic agent for obesity. © 2021 Elsevier Inc.

키워드

AdipocyteAMPKCapmatinibIrisinLipogenesisLipolysisDIFFERENTIATIONIRISINAMPKADIPOGENESISFAT
제목
Capmatinib attenuates lipogenesis in 3T3-L1 adipocytes through an adenosine monophosphate-activated protein kinase-dependent pathway
저자
Ahn, Sung HoLee, Hyun JungPyun, Do HyeonKim, Tae JinAbd El-Aty, A.M.Song, Jin-HoShin, Yong KyooJeong, Ji HoonPark, Eon SubJung, Tae Woo
DOI
10.1016/j.bbrc.2021.03.064
발행일
2021-05
유형
Article
저널명
Biochemical and Biophysical Research Communications
553
페이지
30 ~ 36