핵산지문법을 이용한 새삼屬의 종(種) 식별에 관한 연구

초록

Dodder (Cuscuta spp.) is a very important holoparasitic weed in plant quarantine and weed control. In Korea, C. japonica, C. australis and C. chinensis are spontaneous, but recently, C. pentagona, exotic species, was found. Correct identification of the dodder species by morphological characteristics of seed in quarantine is very difficult. Also, species identification of dodders growing in fields is delayed until dodder plants flower. Objective of this experiment was conducted to develop a reliable method of DNA fingerprinting for species identification with seeds and seedlings. The results are summarized as follows. Seeds of five dodders, i.e., C. australis, C. chinensis, C. japonica, C. pentagona and C. reflexa were used for species identification by DNA fingerprinting. Forty one Operon primers and TaKaRa Taq DNA polymerase (5U ㎕-1) were used to be tested. Consequently 36 primer's showed polymorphism in dodder accessions and 18 primers generated the species-specific bands. For the individual variation within a species in dodder seeds the plant samples were collected from the same area was low except for C. pentagona. Thirteen primers among the 18 primers were used for analysis of inter-specific variation. Overall it was considered that the tested accessions were classified into 4 groups; group A of C. pentagona (Namwon, Daejeon, Jeonju) and C. australis (Hwasun), group B of C. pentagona (Damyang1, Damyang2, Chonan, Yangpyong, Chilgok, Suwon) and C. chinensis (Yeosu), group C of C. japonica (Yeosu, Yongju, China), and group D of C. reflexa (China). The C. japonica-specific 7 bands, the C. chinensis-specific 5 bands, the C. reflexa-specific 7 bands and the C. pentagona-specific 2 bands were identified. As a result, two Operon primers, OPS08 and OPS09, showed sufficient availability for species identification of different dodders.

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