Phycobium rhodophyticola gen. nov., sp. nov. and Aliiphycobium algicola gen. nov., sp. nov., isolated from the phycosphere of marine red algae

초록

Two Gram-stain-negative, strictly aerobic, non-motile, rod-shaped bacteria, designated D3-12ᵀ and G2-2ᵀ, were isolated from the phycosphere of marine red algae. Both strains exhibitedcatalase- and oxidase-positive activities. Strain D3-12ᵀ grew optimally at 30°C, pH 7.0, and2.0–3.0% (w/v) NaCl, while strain G2-2ᵀ showed optimal growth at 30°C, pH 7.0, and 2.0%NaCl. Ubiquinone-10 was the sole respiratory quinone in both strains. The major fatty acids(> 5%) in strain D3-12ᵀ were feature 8 (C18:1 ω7c and/or C18:1 ω6c), 11-methyl-C18:1 ω7c, andC16:0, while strain G2-2ᵀ contained summed feature 8 and C16:0. The predominant polar lipidsin strain D3-12ᵀ were phosphatidylcholine, phosphatidylglycerol, and phosphatidylethanolamine,whereas strain G2-2ᵀ contained phosphatidylglycerol and diphosphatidylglycerol. The genomic DNA G + C content was 59.9% for strain D3-12ᵀ and 60.2% for strain G2-2ᵀ. Phylogenetic analyses based on 16S rRNA and whole-genome sequences placed bothstrains into distinct lineages within the family Roseobacteraceae, separate from previouslydescribed genera. Genome-based relatedness metrics, including average nucleotide identity,digital DNA-DNA hybridization, average amino acid identity, and percentage of conservedproteins, further confirmed that these strains represent novel genera. Based on phenotypic,chemotaxonomic, and molecular characteristics, strains D3-12ᵀ and G2-2ᵀ are proposedas novel genera: Phycobium rhodophyticola gen. nov., sp. nov. (D3-12ᵀ = KACC22712ᵀ = JCM 35528ᵀ) and Aliiphycobium algicola gen. nov., sp. nov. (G2-2ᵀ = KACC 22602ᵀ= JCM 35752ᵀ). Additionally, metabolic features relevant to interactions with marine algae,including genes associated with carbohydrate-active enzymes, vitamin biosynthesis, phenylaceticacid production, and bacterioferritin synthesis, were bioinformatically investigated.

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