Single yeast cell traps for monitoring chromosome dynamics

초록

Movement of chromosome plays a significant role in chromosomal process during the cell cycle. However, analyzing the movement of chromosome is still limited by technical constraints of cell trapping, cultivation, oxygenation, and in situ imaging. Here, we demonstrate a microfluidic based single yeast cell traps to monitor the chromosome dynamics in single living cells for a defined period of time. We prove the basis for dynamic chromosome movement in single yeast cell; telomere/nuclear envelope complex attach to and move in concert with nucleus actin cable.

키워드