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초록
We evaluated genomic fingerprinting procedure based on amplified fragment length polymorphism (AFLP) to investigate genetic relatedness and it's application with seventeen strains of Bacillus anthracis and closely related species. On the basis of AFLP fingerprints, all tested strains of B. anthracis were nearly identical, clustering together at a Jaccard similarity coefficient of 92% (cophenetic value, 0.9689), and could be divided into 6 different closely related species. Therefore, AFLP genomic fingerprinting techniques could be used as rapid, reliable, reproducible and highly discriminatory screening techniques to determine the taxonomic diversity and phylogenetic structure of B. anthracis populations.
키워드
Amplified fragment length polymorphism; Bacillus anthracis; amplified fragment length polymorphism; article; Bacillus anthracis; bacterium identification; genome imprinting; nonhuman; phylogeny; reliability; reproducibility; screening; Bacillus anthracis; Bacteria (microorganisms)
- 제목
- Amplified Fragment Length Polymorphism에 의한 Bacillus anthracis의 분자분류 및 동정
- 제목 (타언어)
- Identification and molecular taxonomy of Bacillus anthracis based on amplified fragment length polymorphism
- 저자
- 김원용; 이은구; 송미옥; 남지연; 박철민; 김기정; 정상인; 최철순
- 발행일
- 2001-03
- 유형
- Article
- 권
- 31
- 호
- 1
- 페이지
- 21 ~ 28
- 언어
- KOR
- 출판사
- The Korean Society for Mocrobiology / The Korean Society of Virology
- 발행국가
- 대한민국
- 분량
- 8 페이지
- ISSN
- E 2093-0429
P 1598-2467