Effects of dietary supplementation with probiotics and their cell-free supernatants on the growth performance and microbial safety of mealworms (Tenebrio molitor)

초록

Edible insects, such as mealworms (Tenebrio molitor), have gained attention as sustainable protein sources due to their minimal resource requirements for cultivation and high nutritional value. This study evaluated the effects of dietary supplementation with probiotics (Lacticaseibacillus, Lactobacillus, and Bifidobacterium) and their cell-free supernatants (CFSs) on the growth and microbial safety of mealworms. Results showed that both probiotics and CFSs significantly enhanced growth performance and survival rates (p < 0.05). Notably, Bifidobacterium bifidum BGN4 yielded the highest weight gain (192.1 mg), length gain (20.0 mm), and specific growth rate (9.2%), while reducing mortality to 3.3%. Dietary supplementation also increased lactic acid bacteria populations and inhibited indicator microorganisms, including aerobic bacteria, coliforms, and yeasts and molds. Furthermore, four Bifidobacterium CFSs eliminated Listeria monocytogenes within 7 days. These findings highlight the probiotics and their CFSs effectively improve mealworm growth performance and microbial safety, providing an improved approach to edible insect production.

키워드