ScholarWorks@중앙대학교

초록

The genus Bevemovirus (family Potyviridae) currently comprises one recognized species, bellflower veinal mottle virus (BVMoV), and one putative member, Striga-associated poty-like virus 1 (SaPlV1). To investigate the hidden diversity of this genus, we mined publicly available plant transcriptome datasets, yielding nine bevemovirus genome sequences from diverse host species, including five Oreocharis species, Campanula takesimana, Ariocarpus retusus, Maihueniopsis conoidea, and Pachycereus pringlei. Phylogenetic analysis of large polyprotein sequences placed all novel viruses within a well-supported Bevemovirus clade, distinct from other Potyviridae genera. Conserved proteolytic cleavage sites inferred from the polyproteins revealed sequence motifs similar to those of Macluravirus and Bymovirus, although several sites exhibited genus-specific features. All genomes encoded the P3N-PIPO protein via a conserved GA6 transcriptional slippage motif. The resulting PIPO peptides displayed conserved N-terminal regions and variable C-termini. Notably, the virus phylogeny was topologically congruent with that of their host plants, suggesting co-segregation and vertical transmission rather than the horizontal transmission typical of many potyviruses. These findings expand the known diversity of Bevemovirus, provide insights into its conserved genomic features, and suggest a persistent virus-host relationship. Our results underscore the utility of plant transcriptome data for virus discovery and for inferring evolutionary processes in RNA virus lineages. Copyright © 2025 Elsevier B.V. All rights reserved.

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