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Deformable l-shaped microwell array for trapping pairs of heterogeneous cells
- Lee, G.H.;
- Kim, S.H.;
- Kang, A.R.;
- Takayama, S.;
- Lee, S.H.;
- ... Park, J.Y.
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SCOPUS
0초록
We propose an L-shaped microwell (L-microwell) array that relies on the elasticity of a polydimethylsiloxane (PDMS) substrate for trapping and pairing heterogeneous cells. We designed an L-microwell suitable for trapping single cell in each branch via stretching/releasing the PDMS substrate, and also performed 3D time-dependent diffusion simulations to visualize how cell secreted molecules diffuse in the L-microwell and communicate with the partner cell. We produced over 5000 cell pairs in one 2.25 cm2 array with about 30 000 L-microwells. The proposed L-microwell array offers a versatile and convenient cell pairing method. © 15CBMS-0001.
키워드
Cell pairing; L-shaped microwell; Substrate deformation; Cells; Cytology; Deformation; Polydimethylsiloxane; Silicones; Cell pairings; Micro wells; PDMS substrate; Polydimethylsiloxane (PDMS) substrates; Secreted molecules; Single cells; Substrate deformation; Time-dependent diffusion simulations; Microchannels
- 제목
- Deformable l-shaped microwell array for trapping pairs of heterogeneous cells
- 저자
- Lee, G.H.; Kim, S.H.; Kang, A.R.; Takayama, S.; Lee, S.H.; Park, J.Y.
- 발행일
- 2015-10
- 유형
- Conference Paper
- 저널명
- MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
- 페이지
- 502 ~ 504
- 언어
- ENG
- 출판사
- Chemical and Biological Microsystems Society
- 분량
- 3 페이지
- ISSN
- P 0000-0000