Arenibacterium halophilum gen. nov., sp. nov., a halotolerant bacterium in the family Rhodobacteraceae isolated from a coastal sand dune

초록

A Gram-stain-negative, non-pigmented, non-spore-forming, motile, strictly aerobic bacterial strain, designated CAU 1492(T), was isolated from a coastal sand dune and its taxonomic position was examined using a polyphasic approach. Cells of strain CAU 1492(T) grew optimally at 30 degrees C, pH 7.0 and in 3% (w/v) NaCl. Phylogenetic analysis based on the 16S rRNA gene sequence of CAU 1492(T) showed that it formed a distinct lineage within the family Rhodobacteraceae as a separate deep branch, with 96.8 % or lower sequence similarity values to representatives of the genera Marivita, Donghicola, Sulfitobacter, Marinovum, Phaeobacter, Primorskyibacter, Roseovarius and Aestuariihabitans. Strain CAU 1492(T) was closely related to Marivita geojedonensis DPG-138(T) (96.8%), Donghicola eburneus SW-277(T) (96.7%), Sulfitobacter porphyrae SCM-1(T) (96.7 %), Marinovum algicola FF3(T) (96.6%) and Aestuariihabitans beolgyonensis BB-MW15(T) (96.4%) based on 16S rRNA gene sequences. The major cellular fatty acids of strain CAU 1492(T) were cyclo-C-19:0 omega 8c and summed feature 8 (C-18:1 omega 7c/C-18:1 omega 6c). The polar lipid pattern was composed of phosphatidylglycerol, phosphatidylethanolamine, an unidentified phospholipid and an unidentified aminolipid. The strain contained Q-10 as the sole respiratory quinone. The draft genome of strain CAU 1492(T) was 4.63 Mb with a DNA G+C content of 63.1mol%. The genome includes 4292 protein-coding genes and a five rRNA operons. On the basis of the phenotypic, chemotaxonomic and genomic data, strain CAU 1492(T) represents a novel genus in the family Rhodobacteraceae for which the name Arenibacterium halophilum gen. nov., sp. nov. is proposed. The type strain of Arenibacterium halophilum is CAU 1492(T) (=KCTC 62998(T)=NBRC 113696(T)).

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